Professionals

HAPS Executive
Locked Bag 1
Hunter Region Mail Centre
NSW 2310, Australia

Phone: (02) 4921 4000
Fax: (02) 4921 4400


International Enquiries
Phone: +61 2 4921 4000
Fax: +61 2 4921 4400

Collection Tube Guide

The tubes below are listed in order of draw. This is a guideline only - use clinical judgement.

Specimen Volume

Tube Cap

Tube Type

Determinations

Instructions

Sterile sample
8-10mls / bottle
Blood Cultures Aerobic (1st) Anaerobic Equipment: Use butterfly cannula, vacutainer barrel and adaptor. Alcohol swab site and leave dry
(1-2 minutes) to reduce bacterial count on skin. Use sterile glove if need to re-touch site of insertion.

2ml
6mL

Clot Activator

Immunology, Iron Serology, B12, Ferritin, CRP, pre albumin (pre protein), VDRL, HIV, Hepatitis, Rubella. Serology.
Lithium & Vancomycin drug levels. Some Hormones BHcg, PTH.

If multiple tests are requested, more than one tube will be required.

(refer online handbook)

2.ml
3.5mL

Sodium Citrate

Coagulation studies, PR, APTT, INR, D-Dimer. Clexane, anti Xa levels. (Refer to handbook for all other coagulation tests.) Allow tube to fill to completion.
Gently invert tube to mix.
2ml
4mL
Lithium Heparin Biochemistry, LFT, UEC, Cardiac Enzymes, Lipids, Cholesterol, Glucose, Troponin, Tumour markers, Most hormones TSH, T3, T4; Progesterone; Drug levels (except Lithium) Gently invert tube to mix.
2ml
4mL
EDTA ESR, FBC, Retics, Blood Group/Cross Match, HbAIC, Red Cell Folate, Lead, HLAB27, BNP, Kleihauer, Cyclosporine, Manganese, fragile X, Direct Coombes, Monospot, ,
On ice: Renin, ACTH, Ammonia Kept Dark: Porphyrin
Gently invert tube to mix.

2 mL

Seditainer

NB ESR - upper Hunter and Lower Hunter and Calvary Mater CPCS)

Gently invert tube to mix.

2 mL

Fluoride Oxalate

Glucose, Lactose
Lactate (on ice)
Gently invert tube to mix.

9 mL

ACD

Tissue typing, Lymphocyte markers

Allow tube to fill to completion.
Gently invert tube to mix.

6 mL

Sodium Heparin

Chromosome studies

Gently invert tube to mix.

7 mL

Trace Metal

Trace metals, zinc, etc

Gently invert tube to mix.

Hazard Warning:

Never inject blood into the tube from needle and syringe.

Discard needle, remove tube cap and drip blood down side of tube. Ensure cap secure when replaced.